|Atheris Analytical - Mass spectrometry Biochemistry Protein engineering Metabolic studies|
|Atheris Discovery - Venoms to drugs Venoms database Lead discovery Early development Biomarkers|
Biochemistry & protein chemistry
Through its long term experience with natural compounds and in vivo metabolic studies, Atheris has gained a solid expertise in a wide range of protein chemistry applications. We have proven know how in the extraction, isolation and purification of minute amounts of biomolecules (such as peptides and proteins) from complex mixtures (such as human blood plasma or animal venoms). We like to characteize the molecular structure of these compounds, to synthesize them and to modify them for an improved quality of the end product. We have built up our analytical facilities around our expertise. Our favorite techniques include:
Isolation & purification
We are nicely equipped for nano- to preparative-HPLC and FPLC, immuno- and affinity-purification. A multitude of parameters can be investigated, such as the choice of the stationary phase, size of column, size of particles, flow-rate, temperature, solvents, gradient, pH, etc... Based on our many standard operating procedures available at Atheris, we like to undertake method optimization, method validation and process development. We typically use UV-visible (diode array) and mass spectrometers as detectors:
Like A Rolling Stone ?
Quality & purity control
We have a broad range of analytical tools to establish certificates of analysis (CoA), which often include matching against a reference sample. Atheris also offers impurities identification, isolation and characterization.
A precise determination of the amount of sample available or its concentration is important, even if it is an impurity present in less than 0.1% amounts or a minor component present in complex mixture. At Atheris we adress this key questions using accurate techniques:
From simple identity confirmation to complex structural elucidations of complex biomolecules with post-translational modification (disulfide bonds, glycosylation, phosphorylation, acetylation, amidation, etc.):
Our analytical equipment, once coupled to our climatic chamber, allows us to conduct stability assays and to identify degradation products under a range of conditions:
This allows us to identify and characterize degradation fragments quite easily at an early stage, which is extremely useful to predict the evolution of a compound in its future environment. These techniques are for example widely used to prepare PK-ADME studies in the frame of pre-clinical studies.
We also happily assist our partners through consulting agreements in our domains of excellence.